Invenza Research

Methotrexate (CAS No. 59-05-2), chemically known as (2S)-2-[[4-[[(2,4-Diaminopteridin-6-yl)methyl]methylamino]benzoyl] amino] pentanedioic acid ;N-[4-[[(2,4-Diamino-6-pteridinyl)methyl]methyl amino] benzoyl]-L-glutamic acid ;4-Amino-N10-methylpteroyl glutamic acid ; 4-Amino, is a well-characterized pharmaceutical impurity reference standard associated with Methotrexate. It is widely used by pharmaceutical research laboratories, analytical scientists, contract research organizations (CROs), and quality control laboratories to support impurity identification, chromatographic method development, analytical method validation, and stability studies.

This reference standard plays an important role in ensuring accurate impurity profiling during the development and quality assessment of Methotrexate-based pharmaceutical products. It is suitable for use in analytical techniques such as HPLC, LC-MS, UPLC, and other validated chromatographic methods where reliable impurity characterization is required.

Methotrexate has the molecular formula C20H22N8O5 and a molecular weight of 454.44 g/mol. It is also known by the chemical name (2S)-2-[[4-[[(2,4-Diaminopteridin-6-yl)methyl]methylamino]benzoyl] amino] pentanedioic acid ;N-[4-[[(2,4-Diamino-6-pteridinyl)methyl]methyl amino] benzoyl]-L-glutamic acid ;4-Amino-N10-methylpteroyl glutamic acid ; 4-Amino and is identified by CAS No. 59-05-2.

Researchers and analytical laboratories utilize this impurity reference standard during pharmaceutical development, quality control testing, method transfer, and regulatory compliance activities. Its use helps support the characterization and monitoring of related substances throughout the product lifecycle.